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- Title
molecular Cloning of the Guinea Pig GRO Gene and Its Rapid Expression in the Tissues of Lipopolysaccharide–Injected Guinea Pigs.
- Authors
Yoshimura, Teizo; Takeya, Motohiro; Ogata, Hiroomi; Yamashiro, Shigeo; Modi, William S.; Gillitzer, Reinhard
- Abstract
Background: CXC chemokines, IL–8 and GRO, play a role in the recruitment of neutrophils in the human. The functional orthologues in the rat and mouse are CINC/KC and MIP–2. The lack of IL–8 made these animals less useful to study the role of IL–8 and GRO. Methods: Guinea pig (gp) cDNA libraries were screened for GRO and IL–1β. A gp genomic library was screened with a gpGRO cDNA probe. Expression of gpIL–8, gpGRO, gpTNFα, and gpIL–1β was investigated by Northern analysis and/or by in situ hybridization. Results: Two gpGRO cDNAs, a 3.0–kb gpGRO genomic DNA, and a gpIL–1β cDNA were cloned. gpGRO and gpIL–8 mRNA were detected in different tissues including lungs 1 h after intraperitoneal injection of lipopolysaccharide (LPS) into guinea pigs. gpGRO, gpIL–8, gpTNFα, and gpIL–1β expression peaked at 3 h in the lungs. Both gpGRO and gpIL–8 mRNA were detected in the cells in alveolar spaces and bronchial epithelial cells. However, gpGRO mRNA, but not gpIL–8, was also expressed in endothelial cells and vascular smooth muscle cells. Conclusions: gpGRO and gpIL–8 mRNA rapidly accumulated in the lungs of guinea pigs after LPS injection. Expression of gpIL–8 and gpGRO mRNA appeared to be independent from TNFα– or IL–1β–stimulation in this model. A high level expression of gpGRO in vascular cells suggest an important role of GRO in the sequestration of neutrophils and multi–organ injuries induced by LPS. The guinea pig will provide an excellent model to study the roles of IL–8 and GRO, important inflammatory mediators in the human.
- Subjects
GUINEA pigs; MOLECULAR cloning; CHEMOKINES; CYTOKINES; INFLAMMATION; NEUTROPHILS
- Publication
International Archives of Allergy & Immunology, 1999, Vol 119, Issue 2, p101
- ISSN
1018-2438
- Publication type
Article
- DOI
10.1159/000024184