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- Title
Modulation of Histamine Type II Receptors on CD8+ T Cells by lnterleukin-2 and Cimetidine.
- Authors
Shibata, Masahiko; Hoon, Dave; Okun, Edward; Morton, Donald
- Abstract
CD8+ T cells are known to play a major role in regulating immune functions under normal and desease conditions. In this study a radioligand binding assay was used to quantitate histamine type II (H2) receptors on activated T cells. The objective was to examine the expression of H2 receptors on T cells during activation with interleukin-2 (IL-2) and treatment with cimetidine. Activated suppressor T cells induced by concanavalin A + IL-2 showed a significant (p < 0.01) increase in H2 receptors compared to the control nonactivated T cells. The T cells expressing the H2 receptors were identified as CD8+ cells; those among them that had an enhanced level of H2 receptors were identified as CD25+. Treatment of activated suppressor cells with the H2 receptor antagonist cimetidine at a concentration of 10--5M significantly reduced the number of H2 receptors. Suppressor cells induced by Con A + IL-2 were able to suppress both IgG and IgM production that was reversible with cimetidine. Incubation of lymphocytes with 50 U/ml IL-2 alone in 3-day culture significantly (p < 0.005) enhanced H2 receptor expression. These studies demonstrate that activated suppressor T cells that are CD8+CD25+ have enhanced levels of H2 receptors and can be modulated by cimetidine. Copyright © 1992 S. Karger AG, Basel
- Publication
International Archives of Allergy & Immunology, 1992, Vol 97, Issue 1, p8
- ISSN
1018-2438
- Publication type
Article
- DOI
10.1159/000236227