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- Title
Atrial Natriuretic Peptide Alleviates Cardiovascular and Metabolic Disorders in a Rat Endotoxemia Model: A Possible Role for Its Anti-inflammatory Properties.
- Authors
Mori, Masaki; Yamanashi, Yoshitaka; Kobayashi, Katsuya; Sakamoto, Atsuhiro
- Abstract
Background: Atrial natriuretic peptide (ANP) plays important roles in the regulation of cardiovascular and renal homeostasis. Furthermore, several studies have shown that ANP may have anti-inflammatory activities. We hypothesized that ANP may alleviate cardiovascular and/or metabolic disorders in rats with lipopolysaccharide (LPS)-induced endotoxemia. Methods: In rats anesthetized with pentobarbital, LPS was injected and ANP was continuously infused at 0.15 μg/kg/min. Mean arterial pressure and pulse rate were monitored hourly, and arterial blood gases were analyzed before LPS injection and at 1, 4, and 6 hours after LPS injection. The expression in the rat left ventricle of mRNAs encoding nitric oxide synthase 2 and 3 (iNOS, eNOS), heme oxygenase 1 and 2 (HO-1, 2), tumor necrosis factor α (TNFα), and interleukin (IL)-1β was measured with the real-time reverse transcriptase-polymerase chain reaction. Results: LPS increased the expression of TNFα, IL-1β, iNOS, and HO-1, which was inhibited by infusion of ANP. Furthermore, the LPS-induced decrease in mean arterial pressure was attenuated, and the acid-base imbalance caused by increased lactate production was improved 6 hours after the administration of ANP. Conclusions: Our results suggest that continuous infusion of ANP counteracts the cardiovascular and metabolic disorders associated with endotoxemia, possibly via anti-inflammatory mechanisms.
- Subjects
METABOLIC disorders; ANIMAL models in research; ATRIAL natriuretic peptides; ENDOTOXEMIA; PENTOBARBITAL; LABORATORY rats
- Publication
Journal of Nippon Medical School, 2010, Vol 77, Issue 6, p296
- ISSN
1345-4676
- Publication type
Article
- DOI
10.1272/jnms.77.296