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- Title
Fast and quantitative analysis of branched-chain amino acids in biological samples using a pillar array column.
- Authors
Song, Yanting; Takatsuki, Katsuya; Isokawa, Muneki; Sekiguchi, Tetsushi; Mizuno, Jun; Funatsu, Takashi; Shoji, Shuichi; Tsunoda, Makoto
- Abstract
In this study, a fast and quantitative determination method for branched-chain amino acids (BCAAs), namely leucine, isoleucine, and valine, was developed using a pillar array column. A pillar array column with low-dispersion turns was fabricated on a 20 × 20-mm microchip using multistep ultraviolet photolithography and deep reactive ion etching. The BCAAs were fluorescently labeled with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), followed by reversed-phase separation on the pillar array column. The NBD derivatives of the three BCAAs and an internal standard (6-aminocaproic acid) were separated in 100 s. The calibration curves for the NBD-BCAAs had good linearity in the range of 0.4-20 μM, using an internal standard. The intra- and interday precisions were found to be in the ranges of 1.42-3.80 and 2.74-6.97 %, respectively. The accuracies for the NBD-BCAA were from 90.2 to 99.1 %. The method was used for the analysis of sports drink and human plasma samples. The concentrations of BCAAs determined by the developed method showed good agreements with those determined using a conventional high-performance liquid chromatography system. As BCAAs are important biomarkers of some diseases, these results showed that the developed method could be a potential diagnostic tool in clinical research.
- Subjects
AMINO acid analysis; QUANTITATIVE chemical analysis; MICROFABRICATION; BIOMARKERS; FLUORESCENCE spectroscopy; PHASE separation
- Publication
Analytical & Bioanalytical Chemistry, 2013, Vol 405, Issue 25, p7993
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-013-7034-7