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- Title
Fine genetic mapping and physical delimitation of the lesion mimic gene spotted leaf 5 ( spl5) in rice ( Oryza sativa L.).
- Authors
Xifeng Chen; Jianwei Pan; Jing Cheng; Guanghuai Jiang; Yang Jin; Zhimin Gu; Qian Qian; Wenxue Zhai; Bojun Ma
- Abstract
Spotted leaf 5 ( spl5), a lesion mimic mutant, was first identified in rice ( Oryza sativa L.) japonica cv. Norin8 in 1978. This mutant exhibits spontaneous disease-like lesions in the absence of any pathogens and resistance to rice blast and bacterial blight; however, the target gene has not yet been isolated. In the present study, we employed a map-based cloning strategy to finely map the spl5 gene. In an initial mapping with 100 F2 individuals ( spl5/ spl5) derived from a cross between the spl5 mutant and indica cv. 93-11, the spl5 gene was located in a 3.3-cM region on chromosome 7 using six simple sequence repeat (SSR) markers. In a high-resolution genetic mapping, two F2 populations with 3,149 individuals ( spl5/ spl5) were derived from two crosses between spl5 mutant and two indica cvs. 93-11 and Zhefu802 and six sequence-tagged site (STS) markers were newly developed. Finally, the spl5 gene was mapped to a region of 0.048 cM between two markers SSR7 and RM7121. One BAC/PAC contig map covering these markers’ loci and the spl5 gene was constructed through Pairwise BLAST analysis. Our bioinformatics analysis shows that the spl5 gene is located in the 80-kb region between two markers SSR7 and RM7121 with a high average ratio of physical to genetic distance (1.67 Mb/cM) and eighteen candidate genes. The analysis of these candidate genes indicates that the spl5 gene represents a novel class of regulators controlling cell death and resistance response in plants.
- Subjects
GENE mapping; RICE; GENES; RICE blast disease; CHROMOSOMES; BIOINFORMATICS
- Publication
Molecular Breeding, 2009, Vol 24, Issue 4, p387
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-009-9299-2