We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Construction of a bacteriophage-derived vector with potential applications in targeted drug delivery and cell imaging.
- Authors
Sharifi, Mehdi; Alizadeh, Ali Akbar; Mivehroud, Maryam Hamzeh; Dastmalchi, Siavoush
- Abstract
There is a strong relationship between the dysregulation of epidermal growth factor receptor (EGFR) and the development of epithelial-derived cancers. Therefore, EGFR has usually been considered the desired target for gene therapy. Here, we propose an approach for targeting EGFR-expressing cells by phage particles capable of displaying EGF and GFP as tumor-targeting and reporting elements, respectively. For this purpose, the superfolder GFP-EGF (sfGFP-EGF) coding sequence was inserted at the N-terminus of the pIII gene in the pIT2 phagemid. The capability of the constructed phage to recognize EGFR-overexpressing cells was monitored by fluorescence microscopy, fluorescence-activated cell sorting (FACS), and cell-based ELISA experiments. FACS analysis showed a significant shift in the mean fluorescence intensity (MFI) of the cells treated with phage displaying sfGFP-EGF compared to phage displaying only sfGFP. The binding of phage displaying sfGFP-EGF to A-431 cells, monitored by fluorescence microscopy, indicated the formation of the sfGFP-EGF-EGFR complex on the surface of the treated cells. Cell-based ELISA experiments showed that phages displaying either EGF or sfGFP-EGF can specifically bind EGFR-expressing cells. The vector constructed in the current study has the potential to be engineered for gene delivery purposes as well as cell-based imaging for tumor detection.
- Subjects
TARGETED drug delivery; CELL imaging; BACTERIOPHAGES; EPIDERMAL growth factor receptors; DOSE-response relationship (Radiation)
- Publication
Biotechnology Letters, 2024, Vol 46, Issue 2, p147
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-023-03455-y