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- Title
Purification and characterization of a novel β-carotene-9′,10′-oxygenase from Saccharomyces cerevisiae ULI3.
- Authors
Wei, Tao; Jia, Beilei; Huang, Shen; Yang, Kunpeng; Jia, Chunxiao; Mao, Duobin
- Abstract
Objectives: A novel β-carotene-9,10′-oxygenase (ScBCO2) has been characterized from Saccharomyces cerevisiae ULI3 to convert β-carotene to β-apo-10′-carotenal, which is a precursor of the plant hormone strigolactone. Results: The ScBCO2 enzyme was purified to homogeneity by ammonium sulfate precipitation, Q sepharose and Superdex-200 chromatography. The molecular mass of the enzyme was ~50 kDa by SDS-PAGE. The purified ScBCO2 enzyme displayed optimal activity at 45 °C and pH 8. Tween 20 (1%, w/v), Trition X-100 (1%, w/v), Mg (5 mM), Zn (5 mM) Cu (5 mM), Ca (5 mM) or DTT (5 mM) increased in the activity by 3, 7, 14, 17, 23, 26 and 27%, respectively. ScBCO2 only exhibited cleavage activity towards carotenoid substrates containing two β-ionone rings and its catalytic efficiency ( kcat/Km) followed the order β-carotene > α-carotene > lutein. Conclusion: ScBCO2 could be used as a potential candidate for the enzymatic biotransformation of β-carotene to β-apo-10′-carotenal in biotechnological applications.
- Subjects
BIOTECHNOLOGY research; BETA carotene; OXYGENASES; SACCHAROMYCES cerevisiae; BIOTRANSFORMATION (Metabolism)
- Publication
Biotechnology Letters, 2015, Vol 37, Issue 10, p1993
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-015-1872-7