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- Title
Spinner-flask culture induces redifferentiation of de-differentiated chondrocytes.
- Authors
Tae-Jin Lee; Suk Ho Bhang; Wan-Guen La; Hee Seok Yang; Jun Yeop Seong; Haeshin Lee; Gun-Il Im; Soo-Hong Lee; Byung-Soo Kim
- Abstract
Implantation of chondrocytes isolated from patients and expanded in number in vitro is being used to treat patients with cartilage injuries. However, chondrocytes de-differentiate during culture with several passages, and cartilage regenerated by implantation of de-differentiated chondrocytes may be suboptimal. Here, we show that a spinner-flask culture system induces formation of chondrocyte aggregates and redifferentiate de-differentiated chondrocytes. Spinner-flask cultures induced the aggregate formation of chondrocytes with passage 1 or 4. Importantly, spinner-flask cultures induced redifferentiation of the de-differentiated chondrocytes, as type I collagen expression was significantly lower and type II collagen expression was significantly higher in spinner flask-cultured chondrocytes than in monolayer-cultured chondrocytes. This system is easily scalable and could be feasible for clinical setting.
- Subjects
CULTURE media (Biology); TISSUE culture; CARTILAGE cells; CARTILAGE injuries; REGENERATION (Biology)
- Publication
Biotechnology Letters, 2011, Vol 33, Issue 4, p829
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-010-0488-1