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- Title
Constructing recombinant herpesvirus BAC vectors with mating-assisted genetically integrated clone method.
- Authors
Sijing Jiang; Xing Zhong; Chao Zhai; Liang Chen; Lixin Ma; Meilin Jin; Huanchun Chen
- Abstract
The large capacity of pseudorabies virus (PRV) for foreign DNA and broad host range make it a prospective tool for the preparation of vaccines and agents of gene and tumour therapy. Here we introduced a cloning strategy that facilitates construction of recombinant PRV–BAC vectors based on mating-assisted genetically integrated clone (MAGIC). The target gene was cloned into a small conditionally replicating donor plasmid, followed by shuffling to a recipient PRV–BAC plasmid in vivo of Escherichia coli through MAGIC. The average efficiency of successful clones was 89%. Moreover, permanent integration of unwanted sequences was avoided.
- Subjects
HERPESVIRUS diseases; DNA; TUMORS; VACCINATION; GENES
- Publication
Biotechnology Letters, 2010, Vol 32, Issue 7, p903
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-010-0253-5