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- Title
Improved detection of rhamnolipid production using agar plates containing methylene blue and cetyl trimethylammonium bromide.
- Authors
Pinzon, Neissa; Ju, Lu-Kwang
- Abstract
Rhamnolipids, produced predominantly by Pseudomonas aeruginosa, are biosurfactants with important applications. For efficient culture screening according to rhamnolipid productivity, the method using agar plates containing methylene blue (MB) and cetyl trimethylammonium bromide (CTAB) was re-examined. An alternative set-up using a fixed underneath light source and image analysis software improved the detection of the circles formed due to complexation between anionic rhamnolipids and cationic MB/CTAB. The roles and effects of MB and CTAB concentrations and pH on the complexation phenomena are reported.
- Subjects
METHYLENE blue; PSEUDOMONAS aeruginosa; BIOSURFACTANTS; LIGHT sources; IMAGE analysis
- Publication
Biotechnology Letters, 2009, Vol 31, Issue 10, p1583
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-009-0049-7