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- Title
Production of d-tagatose at high temperatures using immobilized Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana.
- Authors
Young-Ho Hong; Dong-Woo Lee; Sang-Jae Lee; Eun-Ah Choe; Seong-Bo Kim; Yoon-Hee Lee; Chan-Ick Cheigh; Yu-Ryang Pyun
- Abstract
Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t 1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.
- Subjects
ESCHERICHIA; ESCHERICHIA coli; ISOMERASES; CIS-trans-isomerases; ALGINATES; MICROBIAL polysaccharides
- Publication
Biotechnology Letters, 2007, Vol 29, Issue 4, p569
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-006-9277-2