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- Title
Hyperpolarized [U-<sup>2</sup>H, U-<sup>13</sup>C]Glucose Reports on Glycolytic and Pentose Phosphate Pathway Activity in EL4 Tumors and Glycolytic Activity in Yeast Cells.
- Authors
Timm, Kerstin N.; Hartl, Johannes; Keller, Markus A.; Hu, De‐En; Kettunen, Mikko I.; Rodrigues, Tiago B.; Ralser, Markus; Brindle, Kevin M.
- Abstract
Purpose: A resonance at ~181 ppm in the 13C spectra of tumors injected with hyperpolarized [U-2H, U-13C]glucose was assigned to 6-phosphogluconate (6PG), as in previous studies in yeast, whereas in breast cancer cells in vitro this resonance was assigned to 3-phosphoglycerate (3PG). These peak assignments were investigated here using measurements of 6PG and 3PG 13C-labeling using liquid chromatography tandem mass spectrometry (LC-MS/MS) Methods: Tumor-bearing mice were injected with 13C6 glucose and the 13C-labeled and total 6PG and 3PG concentrations measured. 13C MR spectra of glucose-6-phosphate dehydrogenase deficient (zwf1D) and wild-type yeast were acquired following addition of hyperpolarized [U-2H, U-13C]glucose and again 13Clabeled and total 6PGand 3PGwere measured by LC-MS/MS Results: Tumor 13C-6PG was more abundant than 13C-2PG/ 3PG and the resonance at ~181 ppm matched more closely that of 6PG. 13C MR spectra of wild-type and zwf1D yeast cells showed a resonance at ~181 ppm after labeling with hyperpolarized [U-2H, U-13C]glucose, however, there was no 6PG in zwf1D cells. In the wild-type cells 3PG was approximately four-fold more abundant than 6PG Conclusion: The resonance at ~181 ppm in 13C MR spectra following injection of hyperpolarized [U-2H, U-13C]glucose originates predominantly from 6PG in EL4 tumors and 3PG in yeast cells.
- Publication
Magnetic Resonance in Medicine, 2015, Vol 74, Issue 6, p1543
- ISSN
0740-3194
- Publication type
Article
- DOI
10.1002/mrm.25561