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- Title
Transgenic expression of an unedited mitochondrial orfB gene product from wild abortive (WA) cytoplasm of rice ( Oryza sativa L.) generates male sterility in fertile rice lines.
- Authors
Chakraborty, Anirban; Mitra, Joy; Bhattacharyya, Jagannath; Pradhan, Subrata; Sikdar, Narattam; Das, Srirupa; Chakraborty, Saikat; Kumar, Sachin; Lakhanpaul, Suman; Sen, Soumitra
- Abstract
Main conclusion: Over-expression of the unedited mitochondrial orfB gene product generates male sterility in fertile indica rice lines in a dose-dependent manner. Cytoplasmic male sterility (CMS) and nuclear-controlled fertility restoration are widespread developmental features in plant reproductive systems. In self-pollinated crop plants, these processes often provide useful tools to exploit hybrid vigour. The wild abortive CMS has been employed in the majority of the 'three-line' hybrid rice production since 1970s. In the present study, we provide experimental evidence for a positive functional relationship between the 1.1-kb unedited orfB gene transcript, and its translated product in the mitochondria with male sterility. The generation of the 1.1-kb unedited orfB gene transcripts increased during flowering, resulting in low ATP synthase activity in sterile plants. Following insertion of the unedited orfB gene into the genome of male-fertile plants, the plants became male sterile in a dose-dependent manner with concomitant reduction of ATPase activity of FF-ATP synthase (complex V). Fertility of the transgenic lines and normal activity of ATP synthase were restored by down-regulation of the unedited orfB gene expression through RNAi-mediated silencing. The genetic elements deciphered in this study could further be tested for their use in hybrid rice development.
- Subjects
CYTOPLASMIC male sterility; RICE diseases &; pests treatment; TRANSGENIC plants; RICE; PLANTING; GENOMES
- Publication
Planta: An International Journal of Plant Biology, 2015, Vol 241, Issue 6, p1463
- ISSN
0032-0935
- Publication type
Article
- DOI
10.1007/s00425-015-2269-5