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- Title
Transcriptome analysis of <italic>Brassica juncea</italic> var. <italic>tumida</italic> Tsen responses to <italic>Plasmodiophora brassicae</italic> primed by the biocontrol strain <italic>Zhihengliuella aestuarii</italic>.
- Authors
Luo, Yuanli; Dong, Daiwen; Su, Yu; Wang, Xuyi; Peng, Yumei; Peng, Jiang; Zhou, Changyong
- Abstract
Mustard clubroot, caused by <italic>Plasmodiophora brassicae</italic>, is a serious disease that affects <italic>Brassica juncea</italic> var. <italic>tumida</italic> Tsen, a mustard plant that is the raw material for a traditional fermented food manufactured in Chongqing, China. In our laboratory, we screened the antagonistic bacteria <italic>Zhihengliuella aestuarii</italic> against <italic>P. brassicae.</italic> To better understand the biocontrol mechanism, three transcriptome analyses of <italic>B. juncea</italic> var. <italic>tumida</italic> Tsen were conducted using Illumina HiSeq 4000, one from <italic>B. juncea</italic> only inoculated with <italic>P. brassicae</italic> (P), one inoculated with <italic>P. brassica</italic> and the biocontrol agent <italic>Z. aestuarii</italic> at the same time (P + B), and the other was the control (H), in which <italic>P. brassicae</italic> was replaced by sterile water. A total of 19.94 Gb was generated by Illumina HiSeq sequencing. The sequence data were de novo assembled, and 107,617 unigenes were obtained. In total, 5629 differentially expressed genes between biocontrol-treated (P + B) and infected (P) samples were assigned to 126 KEGG pathways. Using multiple testing corrections, 20 pathways were significantly enriched with Qvalue ≤ 0.05. The resistance-related genes, involved in the production of pathogenesis-related proteins, pathogen-associated molecular pattern-triggered immunity, and effector-triggered immunity signaling pathways, calcium influx, salicylic acid pathway, reactive oxygen intermediates, and mitogen-activated protein kinase cascades, and cell wall modification, were obtained. The various defense responses induced by the biocontrol strain combatted the <italic>P. brassicae</italic> infection<italic>.</italic> The genes and pathways involved in plant resistance were induced by a biocontrol strain. The transcriptome data explained the molecular mechanism of the potential biocontrol strain against <italic>P. brassicae.</italic> The data will also serve as an important public information platform to study <italic>B. juncea</italic> var<italic>. tumida</italic> Tsen and will be useful for breeding mustard plants resistant to <italic>P. brassicae</italic>.
- Subjects
BRASSICA juncea; PLASMODIOPHORA brassicae; BIOLOGICAL control of clubroot; MUSTARD; FOOD fermentation
- Publication
Functional & Integrative Genomics, 2018, Vol 18, Issue 3, p301
- ISSN
1438-793X
- Publication type
Article
- DOI
10.1007/s10142-018-0593-0