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- Title
Novel polymorphisms in the prion protein gene (PRNP) and stability of the resultant prion protein in different horse breeds.
- Authors
Sola, Diego; Artigas, Rody; Mediano, Diego R.; Zaragoza, Pilar; Badiola, Juan José; Martín-Burriel, Inmaculada; Acín, Cristina
- Abstract
Prion diseases are fatal neurodegenerative disorders in which the main pathogenic event is the conversion of the cellular prion protein (PrPC) into an abnormal and misfolded isoform known as PrPSc. Most prion diseases and their susceptibility and pathogenesis are mainly modulated by the PRNP gene that codes for PrP. Mutations and polymorphisms in the PRNP gene can alter PrPC amino acid sequence, leading to a change in transmission efficiency depending on the place where it occurs. Horses are animals that are considered to be highly resistant to prions. Several studies have attempted to identify polymorphisms in the PRNP gene that explain the reason for this high resistance. In this study, we have analysed 207 horses from 20 different breeds, discovering 3 novel PRNP polymorphisms. By using computer programmes such as PolyPhen-2, PROVEAN, PANTHER, Meta-SNP and PredictSNP, we have predicted the possible impact that these new polymorphisms would have on the horse prion protein. In addition, we measured the propensity for amyloid aggregation using AMYCO and analysed the lack of hydrogen bridges that these changes would entail together with their electrostatic potentials using Swiss-PdbViewer software, showing that an increased amyloid propensity could be due to changes at the level of electrostatic potentials.
- Subjects
HORSE breeds; SCRAPIE; HORSE breeding; PRIONS; PRION diseases; AMINO acid sequence; CHRONIC wasting disease
- Publication
Veterinary Research, 2023, Vol 54, Issue 1, p1
- ISSN
0928-4249
- Publication type
Article
- DOI
10.1186/s13567-023-01211-8