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- Title
茶叶抗菌肽粗提物的抑菌活性及其对冷却肉保鲜的影响.
- Authors
王丽芳; 叶良; 谢忠稳; 党向利
- Abstract
To prepare antimicrobial peptide (AMP) extraction from tea and apply it in chilled meat preservation, tea AMP extraction (TAE) was extracted from Longjing tea. Antibacterial activity of TAE was determined by radial diffusion assay. The mechanism of action of TAE against bacteria was analyzed by membrane permeability, phosphorus ions leakage, DNA binding and transmission electron microscopy. The effect of TAE on preservation of chilled meat was also evaluated based on microbial colonies and pH value. The results showed that TAE mainly contained peptides with a molecular weight of less than 18.4 ku, it showed antibacterial activity against Staphylococcus aureus and Escherichia coli, and it could disrupt bacterial cell membrane and enhance cell membrane permeability, resulting in phosphorus ions leakage. However it did not bind to bacterial genomic DNA. TAE could significantly inhibit the growth of bacteria in chilled meat and delay the increasing of pH value, which could prolong the preservation time and had high development and utilization value in food preservation.
- Subjects
ANTIMICROBIAL peptides; BACTERIAL cell membranes; FOOD preservation; MEAT preservation; BACTERIAL DNA; PEPTIDE antibiotics
- Publication
Acta Agriculturae Zhejiangensis, 2022, Vol 34, Issue 10, p2268
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2022.10.21