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- Title
EFFECTS OF TRANSFORMING GROWTH FACTOR-β<sub>1</sub> AND FIBRONECTIN ON SPARC EXPRESSION IN CULTURES OF HUMAN PERIODONTAL LIGAMENT CELLS
- Authors
Fujita, Tsuyoshi; Shiba, Hideki; Sakata, Masatoshi; Uchida, Yuushi; Ogawa, Tetsuji; Kurihara, Hidemi
- Abstract
Transforming growth factor-β1 (TGF-β1) increases synthesis of secreted protein, acidic and rich in cysteine (SPARC), as well as fibronectin (FN) and type I collagen. However, little is known about the regulatory mechanism of SPARC expression. We examined the effect of FN on SPARC expression by TGF-β1 in cultures of human periodontal ligament cells (HPL cells). TGF-β1 increased the SPARC and SPARC mRNA levels in HPL cells. Extracellular matrix (ECM) produced by HPL cells in the presence of TGF-β1 also increased the SPARC levels. Contents of FN and type I collagen in the ECM were increased by TGF-β1. HPL cells cultured on FN-coated plates secreted more SPARC than those on non-coated plates. However, type I collagen had little effect on SPARC levels. The addition of anti-α5 antibody to the cultures abolished the increase in SPARC mRNA expression by TGF-β1. This study demonstrated that FN may be partly involved in the increase in SPARC expression by TGF-β1 in HPL cells.
- Subjects
TRANSFORMING growth factors; FIBRONECTINS
- Publication
Cell Biology International, 2002, Vol 26, Issue 12, p1065
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1006/cbir.2002.0966