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- Title
Optical Study of Lysozyme Molecules in Aqueous Solutions after Exposure to Laser-Induced Breakdown.
- Authors
Sarimov, Ruslan M.; Matveyeva, Tatiana A.; Mozhaeva, Vera A.; Kuleshova, Aleksandra I.; Ignatova, Anastasia A.; Simakin, Alexander V.
- Abstract
The properties of a lysozyme solution under laser-induced breakdown were studied. An optical breakdown under laser action in protein solutions proceeds with high efficiency: the formation of plasma and acoustic oscillations is observed. The concentration of protein molecules has very little effect on the physicochemical characteristics of optical breakdown. After exposure to optical breakdown, changes were observed in the enzymatic activity of lysozyme, absorption and fluorescence spectra, viscosity, and the sizes of molecules and aggregates of lysozyme measured by dynamic light scattering. However, the refractive index of the solution and the Raman spectrum did not change. The appearance of a new fluorescence peak was observed upon excitation at 350 nm and emission at 434 nm at exposure for 30 min. Previously, a peak in this range was associated with the fluorescence of amyloid fibrils. However, neither the ThT assay nor the circular dichroism dispersion confirmed the formation of amyloid fibrils. Probably, under the influence of optical breakdown, a small part of the protein degraded, and a part changed its native state and aggregated, forming functional dimers or "native aggregates".
- Subjects
LYSOZYMES; AQUEOUS solutions; MOLECULAR size; CIRCULAR dichroism; MOLECULES; LASER-induced breakdown spectroscopy; PLASMA oscillations; LIGHT scattering
- Publication
Biomolecules (2218-273X), 2022, Vol 12, Issue 11, p1613
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom12111613