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- Title
Complete genome sequence of S-type Mycobacterium avium subsp. paratuberculosis isolated from an organized goat herd in India.
- Authors
Singh, Vinod Kumar; Das, Chayanika; Kumar, Amit; Prajapati, Malyaj R.; Yadav, Sharad Kumar
- Abstract
The present report communicates the first complete genome sequence of S-type Mycobacterium avium subsp. paratuberculosis, isolated from an organised goat herd in Uttar Pradesh, India. Bacteria were isolated in pure culture on Herrold's egg yolk medium (HEYM) slants containing mycobactin J from the faecal sample collected per-rectally from a clinical diseased goat, and next-generation sequencing (NGS) revealed that the genome sequence length of the isolated strain named MAP-Gt-9 is 4,509,428 bp with no plasmid DNA, with a GC content of 69.5%, an N50 value of 125,474 bp, and an L50 value of 12, containing 4235 coding DNA sequences (CDSs), 44 tRNAs, 3 ncRNAs and 1 each 5S, 16S, 23S rRNA genes.
- Subjects
INDIA; UTTAR Pradesh (India); MYCOBACTERIUM avium paratuberculosis; WHOLE genome sequencing; ANIMAL herds; NUCLEOTIDE sequencing; DNA sequencing; TRANSFER RNA; CATTLE herding; CALPROTECTIN; MOSAIC viruses
- Publication
3 Biotech, 2024, Vol 14, Issue 2, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-023-03896-2