We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Triethyl phosphine decorated cerium oxide nanoparticles exhibit selective killing of the unicellular protozoan parasite Leishmania donovani.
- Authors
Yadav, Nisha; Sharma, Kikku; Sengupta, Souvik; Singh, Sanjay
- Abstract
Globally, Leishmaniasis affects underprivileged communities of the nations and chemotherapy remains one of the preferred treatment options. However, the cytotoxicity, side effects, and cost of the present chemotherapies limit their utilization. Auranofin [an organogold compound having significant structural similarity with triethyl-phosphine (TEP)] has been reported as an effective therapy for Leishmaniasis treatment. Considering the high cost of gold and the strong affinity of cerium oxide nanoparticles (CeNPs) to phosphine ligands, we designed TEP-decorated CeNPs (CeNPs-TEP) and used them as a novel antileishmanial agent. The hydrodynamic size of synthesized CeNPs and CeNPs-TEP was observed to be 22.2 ± 3.7 nm and 92.11 ± 6.2 nm, respectively. CeNPs-TEP provided aqueous stability to TEP as TEP alone is extremely unstable in water. Exposure of CeNPs-TEP showed ~ 60 and ~ 82% cell death in Leishmania donovani Ag83 promastigotes after 24 and 48 h, respectively. The same concentration of CeNPs-TEP did not affect the cellular viability of RAW 264.7 macrophage cells significantly. The oxidative stress and depolarization of the mitochondrial membrane were also observed after the treatment of CeNPs-TEP. Exposure of CeNPs-TEP induced a ~ 2.2-fold increase in ROS generation inside Leishmania donovani Ag83 cells. Dual staining with ethidium bromide and acridine orange reveals that these processes ultimately result in cell death. The results conclude that a combination of CeNPs and TEP could open the door for developing novel antileishmanial therapeutics in the future. Highlights: TEP-decorated CeNPs (CeNPs-TEP) have been synthesized and used as an antileishmanial agent. CeNPs-TEP resulted in a good antileishmanial effect without affecting the cellular viability of RAW 264.7 macrophage cells. ROS generation leads to cell death in Leishmania donovani Ag83 promastigotes.
- Subjects
LEISHMANIA donovani; CERIUM oxides; PHOSPHINE oxides; POOR communities; ACRIDINE orange; PHOSPHINE; CELL death
- Publication
3 Biotech, 2023, Vol 13, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-023-03813-7