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- Title
Establishment and characterization of a new human megakaryoblastic cell line (SET-2) that spontaneously matures to megakaryocytes and produces platelet-like particles.
- Authors
Uozumi, K.; Otsuka, M.; Ohno, N.; Moriyama, T.; Suzuki, S.; Shimotakahara, S.; Matsumura, I.; Hanada, S.; Arima, T.
- Abstract
A new factor-independent megakaryoblastic cell line, designated SET-2, was established from the peripheral blood of a patient with leukemic transformation of essential thrombocythemia (ET). SET-2 expressed CD 4, 7, 13, 33, 34, 36, 38, 41, 61, 71, 117, 126, 130 and c-mpl. In addition, it spontaneously produced numerous platelet-like particles in liquid culture. These particles were shown to be the same size as normal platelets, and to express CD 36, 38, 41, 61 and 71. Proliferation of SET-2 was not influenced by thrombopoietin (TPO) and other hemopoietic cytokines. SET-2 was found to express the platelet-specific proteins such as platelet factor 4 and beta-thromboglobulin. The levels of expression were not altered by TPO. SET-2 also secreted interleukin-6 into the supernatants, as well as normal megakaryocytes. These results suggest that SET-2 spontaneously matures to megakaryocytes and produces platelet-like particles. These findings indicate that SET-2 may be useful for investigating the proliferation and differentiation mechanisms of leukemia cells and the role of c-mpl on megakaryoblasts, megakaryocytes, and platelets in ET. Leukemia (2000) 14, 142-152.
- Subjects
THROMBOCYTOSIS; MEGAKARYOCYTES; BLOOD platelets; LEUKEMIA; ANTIGEN analysis; CELL division; CELL lines; CELLS; COLONY-stimulating factors (Physiology); CYTOKINES; ELECTRON microscopy; FLOW cytometry; IMMUNOPHENOTYPING; INTERLEUKINS; POLYMERASE chain reaction; RNA; REVERSE transcriptase polymerase chain reaction; CANCER cell culture; METABOLISM
- Publication
Leukemia (08876924), 2000, Vol 14, Issue 1, p142
- ISSN
0887-6924
- Publication type
journal article
- DOI
10.1038/sj.leu.2401608