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- Title
Birefringence-induced phase delay enables Brillouin mechanical imaging in turbid media.
- Authors
Antonacci, Giuseppe; Vanna, Renzo; Ventura, Marco; Schiavone, Maria Lucia; Sobacchi, Cristina; Behrouzitabar, Morteza; Polli, Dario; Manzoni, Cristian; Cerullo, Giulio
- Abstract
Acoustic vibrations of matter convey fundamental viscoelastic information that can be optically retrieved by hyperfine spectral analysis of the inelastic Brillouin scattered light. Increasing evidence of the central role of the viscoelastic properties in biological processes has stimulated the rise of non-contact Brillouin microscopy, yet this method faces challenges in turbid samples due to overwhelming elastic background light. Here, we introduce a common-path Birefringence-Induced Phase Delay (BIPD) filter to disentangle the polarization states of the Brillouin and Rayleigh signals, enabling the rejection of the background light using a polarizer. We demonstrate a 65 dB extinction ratio in a single optical pass collecting Brillouin spectra in extremely scattering environments and across highly reflective interfaces. We further employ the BIPD filter to image bone tissues from a mouse model of osteopetrosis, highlighting altered biomechanical properties compared to the healthy control. Results herald new opportunities in mechanobiology where turbid biological samples remain poorly characterized. Non-contact Brillouin microscopy holds potential to disrupt mechanobiology, yet this method faces challenges in presence of strong Rayleigh scattering. Here, the authors introduce a common-path birefringent filter exhibiting ultra-high extinction ratio to investigate turbid biological samples.
- Subjects
BRILLOUIN scattering; POLARIZERS (Light); ACOUSTIC vibrations; INELASTIC scattering; LIGHT scattering; RAYLEIGH scattering
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-49419-2