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- Title
Aberrant activation of TGF-β1 induces high bone turnover via Rho GTPases-mediated cytoskeletal remodeling in Camurati-Engelmann disease.
- Authors
Qi Chen; Yan Yao; Kun Chen; Xihui Chen; Bowen Li; Rui Li; Lidangzhi Mo; Weihong Hu; Mengjie Zhang; Zhen Wang; Yaoping Wu; Yuanming Wu; Fangfang Liu
- Abstract
In the adult skeleton, the bone remodeling process involves a dynamic coordination between osteoblasts and osteoclasts, which is disrupted in diseases with high bone turnover rates and dysregulated transforming growth factor beta 1 (TGF-b1). However, little is known about how TGF-b1 signaling mediates bone resorption. Here, we described a pedigree with a heterozygous variant in TGF-b1 (R218C) that resulted in aberrant activation of TGF-b1 through an activating mechanism that caused Camurati-Engelmann disease (CED). We showed that CED patients have high levels of active Rho GTPases and the migration-related proteins Integrin b1 and Integrin b3 in their peripheral blood. HEK293T cells transfected with a plasmid encoding this mutant expressed high levels of TGF-b1 and active Rho GTPases. Furthermore, activation of Rho by TGF-b1 increased osteoclast formation and bone resorption, with increased migration of pre-osteoclasts, as well as cytoskeletal remodeling of pre-osteoclasts and mature osteoclasts. Importantly, pharmacological inhibition of Rho GTPases effectively rescued hyperactive TGF-b1-induced osteoclastogenesis in vitro. Overall, we propose that Rho GTPases mediate TGF-b1-induced osteoclastogenesis and suggest that Rho-TGF-b1 crosstalk is associated with high bone turnover in CED.
- Subjects
BONE remodeling; TRANSFORMING growth factors-beta; RHO GTPases; BONE resorption; BONE growth; OSTEOBLASTS
- Publication
Frontiers in Endocrinology, 2022, Vol 13, p1
- ISSN
1664-2392
- Publication type
Article
- DOI
10.3389/fendo.2022.913979