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- Title
Skin-homing CD8 + T lymphocytes Show Preferential Growth in vitro and Suppress CD4 + T-cell Proliferation in Patients with Early Stages of Cutaneous T-cell Lymphoma.
- Authors
Thestrup-Pedersen, Kristian; Parhar, Ranjit; Wu, Kaida; Bertilsson, Per-Anders; Meyer, Brian; Abu-Amero, Sayeda; Hainau, Bo; Aleisa, Abdullah; Alfadley, Abdullah; Hamadah, Issam; Alajlan, Abdulmajeed; Al-Hussein, Khalid; Al-Mohanna, Futwan
- Abstract
A total of 27 T-lymphocyte cell strains were established from skin biopsies of 24 patients with Various stages of cutaneous T-cell lymphoma (CTCL) by addition of the T-cell growth factors interleukin (IL)-2 and IL-4. Cellular proliferation and phenotypic changes were measured over 3 months in culture, and T-cell clones were studied using T-cell receptor-y re-arrangement techniques. An average outgrowth of 134 million T lymphocytes from a 4-mm skin biopsy was observed over 2 months. Initially, most T cells expressed the CD4+ phenotype. In 17 cell strains from patients with early CTCL a statistically significant predominance of CD8+ T-lymphocytes developed over 8-weeks' culture, indicating that CD8+ T cells controlled the growth of CD4+ T cells, whereas CD4+ T cells were predominant in cell strains from advanced CTCL (p <0.05). TCR-y re-arrangement studies revealed, on average, 12 T-cell clones per cell strain, which was reduced over time to 6 T-cell clones per cell strain. Lymphocytes from peripheral blood could kill lymphocytes from an autologous cell strain, suggesting the presence of autoreactive cytotoxic T cells. Our study suggests how skin-homing CD8+ T lymphocytes from patients with early stage CTCL can suppress the in vitro growth of skin-homing CD4+ T-lymphocytes, indicating immune surveillance.
- Subjects
LYMPHOCYTES; RETICULOENDOTHELIAL granulomas; LYMPHOPROLIFERATIVE disorders; CELL proliferation; CELL growth; LYMPHOMAS
- Publication
Acta Dermato-Venereologica, 2007, Vol 87, Issue 2, p118
- ISSN
0001-5555
- Publication type
Article
- DOI
10.2340/00015555-0206