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- Title
Stem Cell-Engineered Nanovesicles Exert Proangiogenic and Neuroprotective Effects.
- Authors
Kim, Han Young; Bhang, Suk Ho; Oliveira, Joaquim Miguel
- Abstract
As a tissue regeneration strategy, the utilization of mesenchymal stem cells (MSCs) has drawn considerable attention. Comprehensive research using MSCs has led to significant preclinical or clinical outcomes; however, improving the survival rate, engraftment efficacy, and immunogenicity of implanted MSCs remains challenging. Although MSC-derived exosomes were recently introduced and reported to have great potential to replace conventional MSC-based therapeutics, the poor production yield and heterogeneity of exosomes are critical hurdles for their further applications. Herein, we report the fabrication of exosome-mimetic MSC-engineered nanovesicles (MSC-NVs) by subjecting cells to serial extrusion through filters. The fabricated MSC-NVs exhibit a hydrodynamic size of ~120 nm, which is considerably smaller than the size of MSCs (~30 μm). MSC-NVs contain both MSC markers and exosome markers. Importantly, various therapeutic growth factors originating from parent MSCs are encapsulated in the MSC-NVs. The MSC-NVs exerted various therapeutic effects comparable to those of MSCs. They also significantly induced the angiogenesis of endothelial cells and showed neuroprotective effects in damaged neuronal cells. The results collectively demonstrate that the fabricated MSC-NVs can serve as a nanosized therapeutic agent for tissue regeneration.
- Subjects
EXOSOMES; NEUROPROTECTIVE agents; MESENCHYMAL stem cells; TREATMENT effectiveness; GROWTH factors
- Publication
Materials (1996-1944), 2021, Vol 14, Issue 5, p1078
- ISSN
1996-1944
- Publication type
Article
- DOI
10.3390/ma14051078