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- Title
Effect of beta-sitosterol on carbohydrate metabolic enzymes in liver of high-fat diet and sucrose-induced diabetic rats.
- Authors
Saravanan, R.; Ponnulakshmi, R.; Anandhi, D.; Valli, G.; Girija, S.; Revathy, K.; Ramajayam, G.; Jayaraman, Selvaraj
- Abstract
Aim: The study was aimed at assessing the effect of β-sitosterol on carbohydrate metabolic enzymes in high-fat diet and sucrose-induced type-2 diabetic rats. Experimental design: Adult male Wistar rats were used and divided into four groups: Group I: Control; Group II: Type-2 diabetic rats; Group III: Type-2 diabetic rats treated with β-sitosterol (20 mg/kg b.wt/day, orally for 30 days); and Group IV: Type-2 diabetic rats treated with metformin (50 mg/kg b.wt/day, orally for 30 days). After 30 days of treatment, animals were anesthetized; liver tissues were dissected out and used for the assessment of glucokinase, glucose-6-phosphatase, glycogen synthase, glycogen phosphorylase, and phosphoenolpyruvate carboxykinase (PEPCK) enzyme activities which were studied by spectrophotometric analysis. Results: The activity of glucose-6-phosphatase, glycogen phosphorylase, and PEPCK was significantly raised (P < 0.05) in type-2 diabetic rats. Conversely, glucokinase and glycogen synthase activities were significantly reduced (P < 0.05) in type-2 diabetic rats. Oral administration of the plant sterol (β-sitosterol) could effectively normalize the detrimental changes caused by high diet induction to that of the control and standard drug metformin. Conclusion: The present study shows that β-sitosterol regulates carbohydrate metabolic enzymes in the liver, thereby β-sitosterol may have therapeutic potential candidate for the management of type-2 diabetes.
- Subjects
LIVER enzymes; HIGH-fat diet; GLYCOGEN phosphorylase; CARBOHYDRATES; PHOSPHORYLASES; RATS
- Publication
Drug Invention Today, 2020, Vol 13, Issue 2, p242
- ISSN
0975-7619
- Publication type
Article