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- Title
Validation of Cefpodoxime proxetil and Ambroxol hydrochloride by reverse-phase high-performance liquid chromatographic method.
- Authors
Namratha, Sunkara; Vijayalakshmi, A.
- Abstract
Objective: A simple, accurate, precise reverse-phase high-performance liquid chromatographic method was developed for simultaneous estimation of Cefpodoxime proxetil and hydrochloride HCl in tablet dosage form. Methods: Chromatogram was run through Phenomenex C18 (250 × 4.6 mm, 5 ƒÊm) with a mobile phase consisting acetonitrile:methanol:water (30:30:40 v/v/v) at a flow rate 1 ml/min, 0.025M potassium dihydrogen phosphate as buffer, and pH 4.0 with orthophosphoric acid. UV-spectrometry detection was measured at 245 nm. The developed method was validated in terms of accuracy, linearity, precision, limit of detection (LOD), limit of quantification, and solution stability. Results: The retention time of Cefpodoxime and Ambroxol HCl was found to be 2.35 min and 4.37 min and % RSD of Cefpodoxime proxetil and Ambroxol HCl was found to be 1.02% and 0.28%, respectively. The described method shows good linearity range of 30.150 ƒÊg/ml Cefpodoxime proxetil and 20.100 ƒÊg/ml for Ambroxol HCl. The correlation coefficient of Cefpodoxime proxetil and Ambroxol HCl was 0.999 and 0.999. The LOD was found to be 2.97 ppm and 3.04 ppm and the limit of quantitation values were 10.1 ppm and 10 ppm, respectively. % assay was obtained as 99.6% and 101.4 % for Cefpodoxime proxetil and Ambroxol HCl. Conclusion: The results of the study showed that the proposed reverse-phase high-performance chromatographic method was simple, rapid, precise, and accurate which is useful in the routine determination of Cefpodoxime proxetil and Ambroxol HCl bulk drug and its pharmaceutical dosage form.
- Subjects
REVERSE phase liquid chromatography; ACETONITRILE; DRUG dosage; DRUG tablets; DIHYDROGEN bonding
- Publication
Drug Invention Today, 2018, Vol 10, Issue 2, p174
- ISSN
0975-7619
- Publication type
Article