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- Title
Evidence for renal glomerular receptors for angiotensin II.
- Authors
Sraer, Jean D.; Sraer, Josée; Ardaillou, Raymond; Mimoune, Odile
- Abstract
Monoiodinated angiotensin II (2000 mCi/μmole) was found to bind specifically to isolated rat glomeruli. Equilibrium was reached after 12 min and specific binding represented more than 95% of total binding. Dissociaton after addition of an excess of unlabelled molecules was rapid. The K₁ and k-1 association and dissociation constants determined form time-course studies were 0.254±0.078 × 1010 M-1 min-1 and 0.102±0.018 min-1, respectively and the ratio k-1/k₁ (KD) was 4.51±0.55 × 10-11 M. Angiotensin II, liberated from glomerular binding sites at low pH, retained the ability to bind to fresh membranes. Angiotensin II, angiotensin I, ileu8-angiotensin II and sarc1-ileu8-angiotensin II were all equally effective as competitive inhibitors of 125I-angiotensin II concentration according to a sigmoidal effect vs. long dose curve and the calculated KD (6×10-11 M) was similar to that obtained from binding studies. Specific binding of angiotension II at physiological plasma concentration to rat glomeruli and correlation of this binding with glomerular vasoreactivity suggest a physiological role for this hormone in regulation of glomerular filtration.
- Subjects
KIDNEY glomerulus; ANGIOTENSIN II; MOLECULES; BINDING sites; OLIGOPEPTIDES; PEPTIDE hormones
- Publication
Kidney International, 1974, Vol 6, Issue 1, p241
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1974.105