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- Title
Antimicrobial efficacy of gutta-percha supplemented with cetylpyridinium chloride.
- Authors
Masafumi Tomino; Keiji Nagano; Tatsuhide Hayashi; Kenjiro Kuroki; Tatsushi Kawain; Tomino, Masafumi; Nagano, Keiji; Hayashi, Tatsuhide; Kuroki, Kenjiro; Kawai, Tatsushi
- Abstract
To develop a root canal filling material with high antimicrobial activity, we prepared gutta-percha supplemented with the cationic surfactant cetylpyridinium chloride (CPC). Thermoplastic gutta-percha was supplemented with 0.05%, 0.2%, or 0.8% CPC. The gutta-percha containing CPC was tightly packed at the bottom of a 24-well plate. Its antimicrobial activity against eight representative endodontic pathogens-including gram-positive and gram-negative bacteria and fungi-was evaluated by adding 0.5 mL of liquid samples containing pathogens to the wells. After 24 h of cultivation under appropriate conditions, microbial growth was analyzed by counting colony-forming units (CFU). Gutta-percha alone (without CPC) partially inhibited microbial growth, probably through the antimicrobial effect of some of its components, such as zinc oxide. Addition of CPC dose-dependently increased the antimicrobial efficacy of gutta-percha. Addition of 0.05%, 0.2%, and 0.8% CPC reduced the viable microbial number to below the lower limit of detection (20 CFU/mL) for all tested pathogens except Pseudomonas aeruginosa, which was detected in 0.8% CPC-containing gutta-percha, although the viable number significantly decreased. Gutta-percha with CPC might be useful for preventing microbial infections during root canal therapy. (J Oral Sci 58, 277-282, 2016).
- Subjects
ANTI-infective agents; GUTTA-percha; CETYLPYRIDINIUM chloride; DENTAL fillings; CATIONIC surfactants; ENDODONTICS; COLONY-forming units assay; DENTAL materials; DRUG synergism; MICROBIAL sensitivity tests; PYRIDINE
- Publication
Journal of Oral Science, 2016, Vol 58, Issue 2, p277
- ISSN
1343-4934
- Publication type
journal article
- DOI
10.2334/josnusd.15-0620