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- Title
<sup>68</sup>Ga-DOTA chelate, a novel imaging agent for assessment of myocardial perfusion and infarction detection in a rodent model.
- Authors
Autio, Anu; Uotila, Sauli; Kiugel, Max; Kytö, Ville; Liljenbäck, Heidi; Kudomi, Nobuyuki; Oikonen, Vesa; Metsälä, Olli; Helin, Semi; Knuuti, Juhani; Saraste, Antti; Roivainen, Anne
- Abstract
<bold>Background: </bold>Magnetic resonance imaging (MRI) with Gadolinium 1,4,7,10-tetraazacyclododecane-N',N″,N''',N″″-tetraacetic acid (Gd-DOTA) enables assessment of myocardial perfusion during first-pass of the contrast agent, while increased retention can signify areas of myocardial infarction (MI). We studied whether Gallium-68-labeled analog, 68Ga-DOTA, can be used to assess myocardial perfusion on positron emission tomography/computed tomography (PET/CT) in rats, comparing it with 11C-acetate.<bold>Methods: </bold>Rats were studied with 11C-acetate and 68Ga-DOTA at 24 hours after permanent ligation of the left coronary artery or sham operation. One-tissue compartmental models were used to estimate myocardial perfusion in normal and infarcted myocardium. After the PET scan, hearts were sectioned for autoradiographic detection of 68Ga-DOTA distribution.<bold>Results: </bold>11C-acetate PET showed perfusion defects and histology showed myocardial necrosis in all animals after coronary ligation. Kinetic modeling of 68Ga-DOTA showed significantly higher k1 values in normal myocardium than in infarcted areas. There was a significant correlation (r = 0.82, P = 0.001) between k1 values obtained with 68Ga-DOTA and 11C-acetate. After 10 minutes of tracer distribution, the 68Ga-DOTA concentration was significantly higher in the infarcted than normal myocardium on PET imaging and autoradiography.<bold>Conclusions: </bold>Our results indicate that acute MI can be detected as reduced perfusion, as well as increased late retention of 68Ga-DOTA.
- Subjects
MYOCARDIAL perfusion imaging; MYOCARDIAL infarction; AUTORADIOGRAPHY; POSITRON emission tomography; MAGNETIC resonance imaging; RODENTS; COMPUTED tomography; BIOLOGICAL models; RESEARCH; MYOCARDIUM; HETEROCYCLIC compounds; HEART; PARTICLE accelerators; ANIMAL experimentation; RESEARCH methodology; RADIOISOTOPES; RADIOGRAPHY; CONTRAST media; MEDICAL cooperation; EVALUATION research; GALLIUM isotopes; RATS; COMPARATIVE studies; HEART function tests; RADIOPHARMACEUTICALS
- Publication
Journal of Nuclear Cardiology, 2020, Vol 27, Issue 3, p891
- ISSN
1071-3581
- Publication type
journal article
- DOI
10.1007/s12350-019-01752-6