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- Title
Chemoprevention by Probiotics During 1,2-Dimethylhydrazine-Induced Colon Carcinogenesis in Rats.
- Authors
Walia, Sohini; Kamal, Rozy; Dhawan, D. K.; Kanwar, S. S.
- Abstract
<bold>Background: </bold>Probiotics are believed to have properties that lower the risk of colon cancer. However, the mechanisms by which they exert their beneficial effects are relatively unknown.<bold>Aim: </bold>To assess the impact of probiotics in preventing induction of colon carcinogenesis in rats.<bold>Methods: </bold>The rats were divided into six groups viz., normal control, Lactobacillus plantarum (AdF10)-treated, Lactobacillus rhamnosus GG (LGG)-treated, 1,2-dimethylhydrazine (DMH)-treated, L. plantarum (AdF10) + DMH-treated and L. rhamnosus GG (LGG) + DMH-treated. Both the probiotics were supplemented daily at a dose of 2 × 1010 cells per day. DMH at a dose of 30 mg/kg body weight was administered subcutaneously twice a week for the first 4 weeks and then once every week for a duration of 16 weeks. Glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and catalase as protein expression of genes involved in apoptosis were assessed during DMH-induced colon carcinogenesis in rats.<bold>Results: </bold>DMH treatment decreased the activity of GSH, GPx, GST, SOD and catalase. However, AdF10 and LGG supplementation to DMH-treated rats significantly increased the activity of these enzymes. Further, DMH treatment revealed alterations in the protein expressions of various genes involved in the p53-mediated apoptotic pathway such as p53, p21, Bcl-2, Bax, caspase-9 and caspase-3, which, however, were shifted towards normal control levels upon simultaneous supplementation with probiotics.<bold>Conclusion: </bold>The present study suggests that probiotics can provide protection against oxidative stress and apoptotic-related protein disregulation during experimentally induced colon carcinogenesis.
- Subjects
COLON cancer risk factors; COLON cancer treatment; CHEMOPREVENTION; PROBIOTICS; ANIMAL models of cancer; SUPEROXIDE dismutase; CARCINOGENESIS; COLON tumor prevention; THERAPEUTIC use of probiotics; ANIMAL experimentation; APOPTOSIS; BIOLOGICAL models; CARCINOGENS; COLON tumors; COMPARATIVE studies; LACTOBACILLUS; RESEARCH methodology; MEDICAL cooperation; RATS; RESEARCH; OXIDATIVE stress; EVALUATION research
- Publication
Digestive Diseases & Sciences, 2018, Vol 63, Issue 4, p900
- ISSN
0163-2116
- Publication type
journal article
- DOI
10.1007/s10620-018-4949-z