We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
胚芽乳酸桿菌凍乾保護劑篩選及最適化培養條件之研究.
- Authors
余乘安; 鄭孟純; 劉德華; 蔡宗佑
- Abstract
Lactobacillus plantarum is a species of lactic acid bacteria that exist in food. Usually present in vegetables, dairy, and meat products, they can survive and proliferate in the intestines of humans and other mammals and have the ability to improve intestine flora, reduce low-density cholesterol, and regulate the immune system. This study evaluated the optimal incubation and storage conditions of L. plantarum as reference for further research. First, a growth curve was used to evaluate the growth of L. plantarum. Subsequently, the Box-Behnken design was applied to optimize the growth condition. The response surface method was utilized to investigate the variable combinations of time, temperature, and pH of the medium. These three variables and three levels were central to the rotatable response surface design. The bacteria number, bacteria weight, pH value of the medium, and production of lactic acid were taken as the targets. Lastly, different ratios of soymilk, skim milk, trehalose, and maltodextrin mixed with lactic acid bacteria were freeze-dried to evaluate survival ability. The results showed the log phase of bacteria growth between 6 to 12 h and revealed the highest bacteria number (9.23 ± 0.10 log CFU/mL) and lactic acid production (1.54%) at 24 h. The response surface results showed the best incubation conditions were a medium with a pH value of 6.4, a temperature of 33.2°C, and an incubation time of 33.6 h. The subsequent bacteria number is predicted to reach 10.17 log CFU/mL. Under the protection of different ratios of soymilk, skim milk, trehalose, and maltodextrin, the survival ability of bacteria after freezedrying was higher than 96%. The survival ability of the groups with 15% and 30% skim milk and the group with 30% soymilk could reach 100%. With only a 5% addition of trehalose, survival ability may reach 99.35%. As previously reported, this experiment investigated optimized growth conditions and appropriate freeze-dry protectants of L. plantarum and can be used as reference for future application and research.
- Publication
Taiwanese Journal of Agricultural Chemistry & Food Science, 2021, Vol 59, Issue 3, p95
- ISSN
1605-2471
- Publication type
Article
- DOI
10.6578/TJACFS.202109_59(3).0004