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- Title
Cooperative interactions at the SLP-76 complex are critical for actin polymerization.
- Authors
Barda-Saad, Mira; Shirasu, Naoto; Pauker, Maor H.; Hassan, Nirit; Perl, Orly; Balbo, Andrea; Yamaguchi, Hiroshi; Houtman, Jon C. D.; Appella, Ettore; Schuck, Peter; Samelson, Lawrence E.
- Abstract
T-cell antigen receptor (TCR) engagement induces formation of multi-protein signalling complexes essential for regulating T-cell functions. Generation of a complex of SLP-76, Nck and VAV1 is crucial for regulation of the actin machinery. We define the composition, stoichiometry and specificity of interactions in the SLP-76, Nck and VAV1 complex. Our data reveal that this complex can contain one SLP-76 molecule, two Nck and two VAV1 molecules. A direct interaction between Nck and VAV1 is mediated by binding between the C-terminal SH3 domain of Nck and the VAV1 N-terminal SH3 domain. Disruption of the VAV1:Nck interaction deleteriously affected actin polymerization. These novel findings shed new light on the mechanism of actin polymerization after T-cell activation.
- Subjects
ANTIGENS; ACTIN; POLYMERS; T cell receptors; STOICHIOMETRY
- Publication
EMBO Journal, 2010, Vol 29, Issue 14, p2315
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1038/emboj.2010.133