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- Title
Establishment of a pheasant (Phasianus colchicus) spermatogonial stem cell line for the production of interspecies germ line chimeras.
- Authors
Jeong Hyun Kim; Neelesh Sharma; Sung Woo Kim; Simrinder Singh Sodhi; Mrinmoy Ghosh; Nam Eun Kim; Raj Kumar Mongre; Sung Jong Oh; Dong Kee Jeong
- Abstract
SSCs showed strong reactivity for GDNF family receptor alpha1 (GFRα1) marker. Finally, production of germ line chimeras was attempted by transferring pheasant SSCs into recipient embryos. Although final embryo survival was 5.6% (20/354), the initial survival rate was 88% (312/354). To measure the percent transfer of donor SSC to gonads, the pheasant SSCs were labeled with PKH 26 fluorescent dye. We observed 30% donor cells and 9.48% c-kit/CD117-positive cells in the gonads of recipient chickens. Donor SSCs were thus stably engrafted in the recipient gonads. Conclusions: This study showed that SSCs can be used as a tool for the conservation of endangered birds and the production of germ line chimeras. Our findings yield insights into how we may use the pheasant spermatogonial stem cell line for efficient production of interspecies germ line chimeras and ultimately, to the restoration of endangered birds.
- Subjects
RING-necked pheasant; SPERMATOZOA; STEM cell culture; NEUROTROPHINS; RARE birds; EMBRYO transfer
- Publication
Electronic Journal of Biotechnology, 2014, Vol 17, Issue 5, p211
- ISSN
0717-3458
- Publication type
Article
- DOI
10.1016/j.ejbt.2014.07.001