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- Title
An Economical and Simple Bioaffinity Support for the Immobilization and Stabilization of Tomato (Lycopersicon Esculentum) Peroxidase.
- Authors
Matto, Mahreen; Naqash, Sarah; Husain, Qayyum
- Abstract
Ammonium sulphate fractionated tomato proteins were used for the direct immobilization of peroxidase on bioaffinity, Con A-cellulose support. Con A-cellulose bound peroxidase retained 77% of the original activity. The immobilized enzyme showed very high stability against denaturation mediated by heat, pH, organic solvents and inhibitors. Soluble and immobilized peroxidase exhibited maximum activity at 40 °C and pH 6.0. Con A-cellulose bound peroxidase retained 44% and 51% activity against the exposure to 50% DMF and n-propanol for 1 h, respectively; however soluble enzyme showed only 21% and 20% activity under similar exposure to organic solvents, DMF and n-propanol. Immobilized peroxidase retained significantly higher activity against sodium azide and sodium sulphite as compared to soluble enzyme.
- Subjects
AMMONIUM sulfate; PROTEINS; PEROXIDASE; ENZYMES; SOLVENTS
- Publication
Acta Chimica Slovenica, 2008, Vol 55, Issue 3, p671
- ISSN
1318-0207
- Publication type
Article