We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Water extract of <italic>Clinacanthus nutans</italic> leaves exhibits in vitro, ex vivo and in vivo anti-angiogenic activities in endothelial cell via suppression of cell proliferation.
- Authors
Ng, Chin Theng; Fong, Lai Yen; Tan, Jun Jie; Rajab, Nor Fadilah; Abas, Faridah; Shaari, Khozirah; Chan, Kok Meng; Juliana, Fariza; Yong, Yoke Keong
- Abstract
Background: <italic>Clinacanthus nutans</italic> (Burm. f.) Lindau. has traditionally been using in South East Asia countries to manage cancer. However, scientific evidence is generally lacking to support this traditional claim. This study aims to investigate the in vitro, ex-vivo and in vivo effects of <italic>C. nutans</italic> extracts on angiogenesis. Methods: <italic>C. nutans</italic> leaves was extracted with 50–100% ethanol or deionised water at 1% (<italic>w</italic>/<italic>v</italic>). Human umbilical veins endothelial cell (HUVEC) proliferation was examined using MTT assay. The in vitro anti-angiogenic effects of <italic>C. nutans</italic> were assessed using wound scratch, tube formation and transwell migration assays. The VEGF levels secreted by human oral squamous cell carcinoma (HSC-4) cell and HUVEC permeability were also measured. Besides, the rat aortic ring and chick embryo chorioallantoic membrane (CAM) assays, representing ex vivo and in vivo models, respectively, were performed. Results: The MTT assay revealed that water extract of <italic>C. nutans</italic> leaves exhibited the highest activity, compared to the ethanol extracts. Therefore, the water extract was chosen for subsequent experiments. <italic>C. nutans</italic> leaf extract significantly suppressed endothelial cell proliferation and migration in both absence and presence of VEGF. However, the water extract failed to suppress HUVEC transmigration, differentiation and permeability. <italic>C. nutans</italic> water extract also did not suppress HSC-4 cell-induced VEGF production. Importantly, <italic>C. nutans</italic> water extract significantly abolished the sprouting of vessels in aortic rings as well as in chick embryo CAM. Conclusion: In conclusion, these findings reveal potential anti-angiogenic effects of <italic>C. nutans</italic>, providing new evidence for its potential application as an anti-angiogenic agent.
- Subjects
CELL proliferation; ANIMAL experimentation; BIOLOGICAL assay; CELL differentiation; CELL motility; ENDOTHELIUM; EPITHELIAL cells; FETAL membranes; LEAVES; NEOVASCULARIZATION; RATS; SQUAMOUS cell carcinoma; PLANT extracts; VASCULAR endothelial growth factors; FETAL macrosomia; IN vitro studies
- Publication
BMC Complementary & Alternative Medicine, 2018, Vol 18, Issue 1, p1
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/s12906-018-2270-1