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- Title
Studies on the purification of polysaccharides separated from Tremella fuciformis and their neuroprotective effect.
- Authors
YAXIANG JIN; XINYU HU; YAN ZHANG; TIANJI LIU
- Abstract
The present study aimed to investigate the protective effect of purified polysaccharides from Tremella fuciformis against glutamate-induced cytotoxicity in differentiated PC12 (DPC12) cells. The aqueous extract of Tremella fuciformis was purified using a DEAE-52 cellulose anion exchange column and a Sepharose G-100 column, respectively. A fraction termed TL04 with a 2,033 kDa molecular weight was obtained. The backbone of TL04 is composed of (1→2)- and (1→4)-linked-mannose and (1→3)-linked-glucans. Results revealed that TL04 treatment improved cell viability and suppressed reactive oxygen species accumulation, lactose dehydrogenase release and caspase-3 activity, and ameliorated mitochondrial abnormal alteration caused by glutamate. TL04 pretreatment enhanced the level of B-cell lymphoma 2 (Bcl-2), and suppressed Bax expression and cytochrome c (Cyto C) release in glutamate-treated cells. Exposure to glutamate strongly increased the activity of caspase-8, caspase-9 and caspase-3, which were significantly reversed by TL04 pretreatment. The presence of Ac-DEVD-CHO (a caspase-3 inhibitor) markedly enhanced the potency of TL04 in improving the viability of glutamate-exposed DPC12 cells. Collectively, the results demonstrated that the purified polysaccharides separated from Tremella fuciformis (TL04) possess a neuroprotective effect against glutamate-induced DPC12 cell damage predominantly through the caspase-dependent mitochondrial pathway. The present study provides an experimental foundation supporting purified TL04 as a potential therapeutic agent for neurodegenerative diseases.
- Subjects
TREMELLA fuciformis; GLUTAMIC acid; MITOCHONDRIA; REACTIVE oxygen species; GLUCANS; POLYSACCHARIDES
- Publication
Molecular Medicine Reports, 2016, Vol 13, Issue 5, p3985
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2016.5026