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- Title
CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant.
- Authors
Wu, Chenshuo; Chen, Zhi; Li, Chaozhou; Hao, Yabin; Tang, Yuxuan; Yuan, Yuxuan; Chai, Luxiao; Fan, Taojian; Yu, Jiangtian; Ma, Xiaopeng; Al-Hartomy, Omar A.; Wageh, S.; Al-Sehemi, Abdullah G.; Luo, Zhiguang; He, Yaqing; Li, Jingfeng; Xie, Zhongjian; Zhang, Han
- Abstract
Highlights: A robust technique-methodology of electrochemical CRISPR sensing is first proposed for the rapid, highly sensitive and specific detection of SARS-CoV-2 variant without any nucleic-acid-amplification assays. Using the DNA template identical to SARS-CoV-2 Delta spike gene sequence as model, our biosensor exhibited excellent analytical detection limit (50 fM) and high linearity (R2 = 0.987) without any amplification assay. Specific crRNA was designed to match the mutation site on nucleic acid sequence of the SARS-CoV-2 Delta variant, presenting programmability, universality, and scalability for diagnosis of other emerging SARS-CoV-2 variants.Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now, which is time-consuming and requires expensive instrumentation, and the confirmation of variants relies on further sequencing techniques. Herein, we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid, highly sensitivity and specificity for the detection of SARS-CoV-2 variant. To enhance the sensing capability, gold electrodes are uniformly decorated with electro-deposited gold nanoparticles. Using DNA template identical to SARS-CoV-2 Delta spike gene sequence as model, our biosensor exhibits excellent analytical detection limit (50 fM) and high linearity (R2 = 0.987) over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification assays. The detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas system. Furthermore, based on the wireless micro-electrochemical platform, the proposed biosensor reveals promising application ability in point-of-care testing.
- Publication
Nano-Micro Letters, 2022, Vol 14, Issue 1, p1
- ISSN
2311-6706
- Publication type
Article
- DOI
10.1007/s40820-022-00888-4