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- Title
Nicotine induces cell proliferation by β-arrestin--mediated activation of Src and Rb--Raf-1 pathways.
- Authors
Dasgupta, Piyali; Rastogi, Shipra; Pillai, Smitha; Ordonez-Ercan, Dalia; Morris, Mark; Haura, Eric; Chellappan, Srikumar
- Abstract
Recent studies have shown that nicotine, a component of cigarette smoke, can stimulate the proliferation of non-neuronal cells. While nicotine is not carcinogenic by itself, it has been shown to induce cell proliferation and angiogenesis. Here we find that mitogenic effects of nicotine in non-small cell lung cancers (NSCLCs) are analogous to those of growth factors and involve activation of Src, induction of Rb-Raf-1 interaction, and phosphorylation of Rb. Analysis of human NSCLC tumors show enhanced levels of Rb-Raf-1 complexes compared with adjacent normal tissue. The mitogenic effects of nicotine were mediated via the α7-nAChR subunit and resulted in enhanced recruitment of E2F1 and Raf-1 on proliferative promoters in NSCLC cell lines and human lung tumors. Nicotine stimulation of NSCLC cells caused dissociation of Rb from these promoters. Proliferative signaling via nicotinic acetylcholine receptors (nAChRs) required the scaffolding protein β-arrestin; ablation of β-arrestin or disruption of the Rb-Raf-1 interaction blocked nicotine-induced proliferation of NSCLCs. Additionally, suppression of β-arrestin also blocked activation of Src, suppressed levels of phosphorylated ERK, and abrogated Rb-Raf-1 binding in response to nicotine. It appears that nicotine induces cell proliferation by β-arrestin-mediated activation of the Src and Rb-Raf-1 pathways.
- Subjects
NICOTINE; CELL proliferation; NEOVASCULARIZATION; NEURONS; LUNG cancer; ACETYLCHOLINE
- Publication
Journal of Clinical Investigation, 2006, Vol 116, Issue 8, p2208
- ISSN
0021-9738
- Publication type
Article
- DOI
10.1172/JCI28164