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- Title
Production of <italic>R</italic>-Mandelic Acid Using Nitrilase from Recombinant <italic>E. coli</italic> Cells Immobilized with Tris(Hydroxymethyl)Phosphine.
- Authors
Zhang, Xin-Hong; Liu, Zhi-Qiang; Xue, Ya-Ping; Wang, Yuan-Shan; Yang, Bo; Zheng, Yu-Guo
- Abstract
Recombinant <italic>Escherichia coli</italic> cells harboring nitrilase from <italic>Alcaligenes faecalis</italic> were immobilized using tris(hydroxymethyl)phosphine (THP) as the coupling agent. The optimal pH and temperature of the THP-immobilized cells were determined at pH 8.0 and 55 °C. The half-lives of THP-immobilized cells measured at 35, 40, and 50 °C were 1800, 965, and 163 h, respectively. The concentration of <italic>R</italic>-mandelic acid (<italic>R</italic>-MA) reached 358 mM after merely 1-h conversion by the immobilized cells with 500 mM <italic>R</italic>,<italic>S-</italic>mandelonitrile (<italic>R</italic>,<italic>S</italic>-MN), affording the highest productivity of 1307 g L−1 day−1 and the space-time productivity of 143.2 mmol L−1 h−1 g−1. The immobilized cells with granular shape were successfully recycled for 60 batches using 100 mM <italic>R</italic>,<italic>S</italic>-MN as substrate at 40 °C with 64% of relative activity, suggesting that the immobilized <italic>E. coli</italic> cells obtained in this study are promising for the production of <italic>R</italic>-MA.
- Subjects
IMMOBILIZED cells; ALCALIGENES faecalis; NITRILASES; ENANTIOSELECTIVE catalysis; HYDROLYSIS
- Publication
Applied Biochemistry & Biotechnology, 2018, Vol 184, Issue 3, p1024
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-017-2604-3