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- Title
CO Biofixation of Actinobacillus succinogenes Through Novel Amine-Functionalized Polystyrene Microsphere Materials.
- Authors
Zhu, Wenhao; Li, Qiang; Dai, Ning
- Abstract
CO-derived succinate production was enhanced by Actinobacillus succinogenes through polystyrene (PSt) microsphere materials for CO adsorption in bioreactor, and the adhesion forces between A. succinogenes bacteria and PSt materials were characterized. Synthesized uniformly sized and highly cross-linked PSt microspheres had high specific surface areas. After modification with amine functional groups, the novel amine-functionalized PSt microspheres exhibited a high adsorption capacity of 25.3 mg CO/g materials. After addition with the functionalized microspheres into the culture broth, CO supply to the cells increased. Succinate production by A. succinogenes can be enhanced from 29.6 to 48.1 g L. Moreover, the characterization of interaction forces between A. succinogenes cells and the microspheres indicated that the maximal adhesive force was about 250 pN. The amine-functionalized PSt microspheres can adsorb a large amount of CO and be employed for A. succinogenes anaerobic cultivation in bioreactor for high-efficiency production of CO-derived succinate.
- Subjects
CARBON sequestration; ACTINOBACILLUS; POLYSTYRENE; MICROSPHERES; ADSORPTION (Biology)
- Publication
Applied Biochemistry & Biotechnology, 2017, Vol 181, Issue 2, p584
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-016-2233-2