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- Title
Longitudinal Use of Micro-computed Tomography Does Not Alter Microarchitecture of the Proximal Tibia in Sham or Ovariectomized Sprague-Dawley Rats.
- Authors
Longo, Amanda; Sacco, Sandra; Salmon, Phil; Ward, Wendy; Longo, Amanda B; Sacco, Sandra M; Salmon, Phil L; Ward, Wendy E
- Abstract
In vivo micro-computed tomography (μCT) provides the ability to measure longitudinal changes to tibia microarchitecture, but the effect of this radiation is not well understood. The right proximal tibia of Sprague-Dawley rats (n = 12/group) randomized to Sham-control (Sham) or ovariectomy (OVX) surgery at 12 weeks of age was scanned using μCT at 13, 17, 21, and 25 weeks of age, at a resolution of 18 μm and a radiation dose of 603 mGy. The left proximal tibia was scanned only at 25 weeks of age to serve as an internal non-irradiated control. Repeated irradiation did not affect tibia microarchitecture in Sham or OVX groups, although there was an increase in cortical eccentricity (P < 0.05). All trabecular outcomes and cortical BMD were different (P < 0.05) between groups after only 1 week post-surgery and differences persisted to study endpoint. Characteristic changes to trabecular bone were observed in OVX rats over time. Interactions of time and hormone status were found for cortical BMD (P < 0.001), Ps. Pm., and Ec. Pm. (P < 0.05). Repeated irradiation of the tibia at 13, 17, 21, and 25 weeks does not cause adverse effects to microarchitecture, regardless of hormone status. This radiation dose can be applied over a typical 3-month study period to comprehensively understand how an intervention alters tibia microarchitecture without confounding effects of radiation.
- Subjects
COMPUTED tomography; TIBIA physiology; RADIATION doses; LABORATORY rats; CANCELLOUS bone; ANIMAL experimentation; BIOLOGICAL models; COMPARATIVE studies; LONGITUDINAL method; RESEARCH methodology; MEDICAL cooperation; OSTEOPOROSIS; OVARIECTOMY; RATS; RESEARCH; TIBIA; EVALUATION research; BONE density
- Publication
Calcified Tissue International, 2016, Vol 98, Issue 6, p631
- ISSN
0171-967X
- Publication type
journal article
- DOI
10.1007/s00223-016-0113-y