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- Title
Bacillus circulans WZ-12—a newly discovered aerobic dichloromethane-degrading methylotrophic bacterium.
- Authors
Wu, S. J.; Zhang, L. L.; Wang, J. D.; Chen, J. M.
- Abstract
A novel dichloromethane (DCM)-degrading bacterial strain named WZ-12 (GenBank accession no. EF100968) was isolated and identified as Bacillus circulans based on standard morphological and physiological properties and nucleotide sequence analysis of enzymatically amplified 16S ribosomal deoxyribonucleic acid. DCM dehalogenase from B. circulans WZ-12 was purified to 8.27-fold with a yield of 34.83%. The electrophoretically homogeneous-purified enzyme exhibited a specific activity of 118.82 U/mg. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis of purified DCM dehalogenase gave a distinct band with an estimated molecular mass of 20,000 ± 1,000.
- Subjects
DICHLOROMETHANE; METHANE; BACILLUS (Bacteria); BIODEGRADATION; BIOCHEMISTRY; NUCLEOTIDE sequence; ELECTROCHEMISTRY
- Publication
Applied Microbiology & Biotechnology, 2007, Vol 76, Issue 6, p1289
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-007-1100-z