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- Title
Isolation and characterization of a cell-associated protein of Bacillus pumilus PH-01.
- Authors
Hong, H.-B.; Chang, Y.-S.; Choi, S.-D.; Nam, I.-H.; Lee, Y.-E.
- Abstract
A cell-associated protein released from Bacillus pumilus PH-01 showed an affinity for some dioxins, like 1,2,3,4-tetrachlorodibenzo-p-dioxin (TCDD) and 1,2,3,4-tetrachlorodibenzofuran (TCDF), and the concentration of the protein increased when B. pumilus PH-01 was boiled in minimal salts medium. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-mass spectrometry revealed that the boiled culture supernatant contained a major protein with a molecular mass of 5,313.4 Da. The adsorption behavior of the protein for 1,2,3,4-TCDD and 1,2,3,4-TCDF was examined by digesting it with proteinase K and trypsin, showing that the proteolyzed protein lost the ability to adsorb the compounds. The amino acid sequence of the protein was determined by automated Edman degradation and tandem mass spectrometry. A search of the protein databases showed no existence of proteins with an homologous sequence.
- Subjects
CELLS; PROTEINS; BACILLUS (Bacteria); BACILLACEAE; BACILLUS anthracis
- Publication
Applied Microbiology & Biotechnology, 2001, Vol 56, Issue 3/4, p402
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s002530100638