We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cloning of the cyclodextrin glucanotransferase gene from alkalophilic Bacillus sp. A2-5a and analysis of the raw starch-binding domain.
- Authors
Ohdan, K.; Kuriki, T.; Takata, H.; Okada, S.
- Abstract
The cyclodextrin glucanotransferase (CGTase) gene of alkalophilic Bacillus sp. A2-5a was cloned and expressed in Bacillus subtilis ANA-1 as a host. The DNA region included an open reading frame encoding a 704-amino-acid polypeptide with a typical raw starch-binding motif in its C-terminal region. The CGTase purified from Bacillus sp. A2-5a bound to raw starch as strongly as porcine pancreas α-amylase, as expected from the sequence motif. A chromosomal region (a DNA fragment of about 14.1 kbp) including the CGTase gene was also cloned and the nucleotide sequence was determined. Possible cyclodextrinase and putative cyclodextrin-binding protein genes were found in the flanking region of the CGTase gene, which implied that the novel starch-degradation pathway postulated for a gram-negative bacterium [Klebsiella oxytoca; Fiedler et al. (1996) J Mol Biol 256: 279–291] also exists in a gram-positive bacterium i.e. Bacillus.
- Subjects
CLONING; CYCLODEXTRINS; GENES; ALKALOPHILIC microorganisms; BACILLUS subtilis
- Publication
Applied Microbiology & Biotechnology, 2000, Vol 53, Issue 4, p430
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s002530051637