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- Title
Development of a screening system for DNA damage and repair of potential carcinogens based on dual luciferase assay in human HepG2 cell.
- Authors
Fan, Longgang; Niu, Yujie; Zhang, Shaohui; Shi, Lei; Guo, Huicai; Liu, Yi; Zhang, Rong
- Abstract
At present, different methods are used for the detection of early biological effects of DNA-damaging agents in environment. Some sensitive testing methods employing DNA damage–inducing genes RNR3, RAD51, RAD54 or growth-arrested and DNA damage–inducible gene 153 (Gadd 153) are used to detect the DNA damage. The host cell reactivation (HCR) assay is a functional assay that is based on the independent transfection of cells with either damaged or undamaged plasmid DNA and allows the identification of the genes responsible for DNA repair-deficient syndromes. In this study, we combined the gadd153-luc test system and HCR assay to measure the DNA damage and DNA repair by dual luciferase assay. We used 16 DNA-damaging agents all of which were detected by a positive dual luciferase reporter test system. The sensitivity of the dual luciferase assay system to detect DNA damage/repair was same as the gadd153-luc test system and/or the HCR assay. Since DNA repair is important to maintain genetic stability, DNA damage and repair have been good biomarkers of early biological effects of DNA-damaging agents. Accordingly, the measurement of DNA repair capacity should be a valued tool in molecular epidemiology studies. The dual luciferase assay described in this study is rapid, convenient, stable and standard.
- Subjects
GENETIC testing; DNA damage; DNA repair; CARCINOGENS; LIVER cells; LUCIFERASES; RAD51 recombinase; RIBOSOMAL RNA
- Publication
Mutagenesis, 2013, Vol 28, Issue 5, p515
- ISSN
0267-8357
- Publication type
Article
- DOI
10.1093/mutage/get028