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- Title
High-performance anion-exchange chromatography with pulsed amperometric detection for carbohydrate analysis of glycoproteins.
- Authors
Rohrer, J.; Basumallick, L.; Hurum, D.
- Abstract
High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is an established technique for the carbohydrate analysis of glycoproteins. HPAE-PAD is routinely used for determinations of monosaccharide, sialic acid, mannose-6-phosphate (M-6-P), and oligosaccharide contents of a glycoprotein. This is true for both the initial investigation of a glycoprotein and routine assays of recombinant therapeutic glycoproteins. This contribution reviews the fundamentals of HPAE-PAD, recent technological improvements, and advances in the last ten years in its application to carbohydrate analysis of glycoproteins. The application areas reviewed include monosaccharide determinations, sialic acid determinations, M-6-P determinations, sugar alcohol determinations, analysis of polysialic acids, neutral and charged oligosaccharide analysis, following glycosidase and glycosyltransferase reactions, and coupling HPAE-PAD to mass spectrometry (MS).
- Subjects
MONOSACCHARIDES; GLYCOPROTEINS; OLIGOSACCHARIDES; SIALIC acids; CARBOHYDRATES; MANNOSE 6-phosphate; TECHNOLOGICAL innovations
- Publication
Biochemistry (00062979), 2013, Vol 78, Issue 7, p697
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S000629791307002X