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- Title
A Novel Broad-Host-Range Phage for Treatment of Mouse Model of Escherichia coli Urinary Tract Infection.
- Authors
Abed, Suhad Y.; Alsakini, Ali H.; Mohammad, Manal K.; Mohammed, Sundus Q.; Kaabi, Sadeq A. G.
- Abstract
The increasing emergence of drug resistance in pathogens of urinary tract infections (UTIs) has resulted in a pressing need to develop new approaches to control this infection. This study is aimed at isolating broad-host-range phage active against isolates of uropathogenic Escherichia coli and other bacterial pathogens causing UTIs and to study its therapeutic potential on mouse model of chronic UTIs. The broad-host-phage was isolated through enrichment of E. coli isolates with sewage water and detected by spot lysis method, growth inhibition assay and top plaque assay. A novel broad-host-range phage, PEC34, was isolated and showed a 100% lytic activity towards UPEC, whereas other isolated phages showed a 12-30% lytic activity. The phage PEC34 showed lytic activity against Gram-negative pathogens of UTI like Proteus mirabilis, Citrobacter freundii, Klebsiella oxytoca and Enterobacter cloacae, but showed no lytic activity against Pseudomonas aeruginosa nor Staphylococcus aureus. The phage therapy of mouse model of chronic UTI by the broad-host-range phage PEC34 through transurethral and intraperitoneal injection of 100 µL (1011 plaque forming units (PFU) of phage preparation on day 10 after the establishment of the infection resulted in clearance of pathogenic bacteria from the urine of mice and homogenates of bladders and kidneys of sacrificed mice after only 24 h, whereas therapy by a narrow host-range phage PEC80 showed no such effects. The phage P34 could be a strong candidate for treatment of UTIs and other infections caused by bacteria sensitive to lytic action of the phage.
- Subjects
TREATMENT of escherichia coli diseases; URINARY tract infections; DRUG resistance in bacteria; CITROBACTER freundii; ENTEROBACTER cloacae; THERAPEUTIC use of bacteriophages
- Publication
Tropical Journal of Natural Product Research, 2022, Vol 6, Issue 4, p488
- ISSN
2616-0684
- Publication type
Article
- DOI
10.26538/tjnpr/v6i4.6