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- Title
Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain.
- Authors
Luchetti, Alessandro; Milani, Davide; Ruffini, Francesca; Galli, Rossella; Falini, Andrea; Quattrini, Angelo; Scotti, Giuseppe; Comi, Giancarlo; Martino, Gianvito; Furlan, Roberto; Politi, Letterio S.
- Abstract
We investigated the potential of antibody-vectorialized superparamagnetic iron oxide (SPIO) particles as cellular specific magnetic resonance contrast agents to image lymphocyte populations within the central nervous system (CNS), with the final goal of obtaining a reliable tool for noninvasively detecting and tracking specific cellular populations in vivo. We used superparamagnetic particles bound to a monoclonal antibody. The particle is the contrast agent, by means of its T2 relaxation properties; the antibody is the targeting vector, responsible for homing the particle to target a surface antigen. To investigate the efficiency of particle vectorialization by these antibodies, we compared two types of antibody-vectorialized CD3-specific particles in vivo. We successfully employed vectorialized SPIO particles to image B220+ cells in a murine model of B-cell lymphoma. Likewise, we were able to identify CD3+ infiltrates in amurinemodel ofmultiple sclerosis. The specificity of the technique was confirmed by immunohistochemistry and electron microscopy of corresponding sections. Our findings suggest that indirect binding of the antibody to a streptavidinated particle allows for enhanced particle vectorialization compared to covalent binding of the antibody to the particle.
- Subjects
MONOCLONAL antibodies; IRON oxides; MAGNETIC resonance imaging; LYMPHOCYTES; IMMUNOHISTOCHEMISTRY; LABORATORY mice; CENTRAL nervous system
- Publication
Molecular Imaging, 2012, Vol 11, Issue 2, p114
- ISSN
1535-3508
- Publication type
Article
- DOI
10.2310/7290.2011.00032