We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes.
- Authors
Wang, Zhongying; Gurlo, Tatyana; Matveyenko, Aleksey V.; Elashoff, David; Wang, Peiyu; Rosenberger, Madeline; Junge, Jason A.; Stevens, Raymond C.; White, Kate L.; Fraser, Scott E.; Butler, Peter C.
- Abstract
Type 2 diabetes is characterized by β and α cell dysfunction. We used phasor-FLIM (Fluorescence Lifetime Imaging Microscopy) to monitor oxidative phosphorylation and glycolysis in living islet cells before and after glucose stimulation. In healthy cells, glucose enhanced oxidative phosphorylation in β cells and suppressed oxidative phosphorylation in α cells. In Type 2 diabetes, glucose increased glycolysis in β cells, and only partially suppressed oxidative phosphorylation in α cells. FLIM uncovers key perturbations in glucose induced metabolism in living islet cells and provides a sensitive tool for drug discovery in diabetes. Using phasor-FLIM (Fluorescence Lifetime Imaging Microscopy), Wang et al. reveal differential usages of oxidative phosphorylation and glycolysis in living islet cells following glucose stimulation. This study suggests the applicability of FLIM as a drug discovery tool as it reports perturbations in glucose metabolism in living islet cells.
- Subjects
TYPE 2 diabetes; CELL metabolism; OXIDATIVE phosphorylation; ISLANDS of Langerhans; BLOOD sugar
- Publication
Communications Biology, 2021, Vol 4, Issue 1, p1
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-021-02113-1